› 1yui

Solution NMR

SOLUTION NMR STRUCTURE OF THE GAGA FACTOR/DNA COMPLEX, REGULARIZED MEAN STRUCTURE

Released:
DOI: 10.2210/pdb1yui/pdb
PDB entry 1yui coloured by chain, front view.
PDB entry 1yui coloured by chain, side view.
PDB entry 1yui coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The solution structure of a specific GAGA factor-DNA complex reveals a modular binding mode.
Omichinski JG, Pedone PV, Felsenfeld G, Gronenborn AM, Clore GM
Nat Struct Biol 4 122-32 (1997)
PMID: 9033593

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114874 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription activator GAGA Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 6.2 KDa
Source organism: Drosophila melanogaster
UniProt:
  • Canonical: Q08605 (Residues: 319-372; Coverage: 9%)
Gene names: Adf-2, CG33261, GAGA, Nc70F, TFGAGA, Trl
Sequence domains: GAGA factor
Structure domains: Classic Zinc Finger
DNA (5'-D(*GP*CP*CP*GP*AP*GP*AP*GP*TP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.38 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*GP*TP*AP*CP*TP*CP*TP*CP*GP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.33 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Not provided

Quick links

Citations

19 review citations

DNA recognition by Cys2His2 zinc finger proteins.
Wolfe et al. (2000)
18 more

7 mentions without citation

Protein-DNA binding specificity predictions with structural models.
Morozov et al. (2005)
6 more