› 1yiu

Solution NMR

Itch E3 ubiquitin ligase WW3 domain

Released:
DOI: 10.2210/pdb1yiu/pdb
PDB entry 1yiu coloured by chain, ensemble of 8 models, front view.
PDB entry 1yiu coloured by chain, ensemble of 8 models, side view.
PDB entry 1yiu coloured by chain, ensemble of 8 models, top view.
Source organism: Mus musculus
Primary publication:
Phosphorylation of either Ser16 or Thr30 does not disrupt the structure of the Itch E3 ubiquitin ligase third WW domain.
Shaw AZ, Martin-Malpartida P, Morales B, Yraola F, Royo M, Macias MJ
Proteins 60 558-60 (2005)
PMID: 15971201

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183943 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Itchy Chain: A
Molecule details ›
Chain: A
Length: 37 amino acids
Theoretical weight: 4.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8C863 (Residues: 399-432; Coverage: 4%)
Gene name: Itch
Sequence domains: WW domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli BL21

Quick links

Citations

4 citation in other articles

The role of the turn in beta-hairpin formation during WW domain folding.
Sharpe et al. (2007)
3 more