› 1xxx

X-ray diffraction
2.28Å resolution

Crystal structure of Dihydrodipicolinate Synthase (DapA, Rv2753c) from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1xxx/pdb
PDB entry 1xxx coloured by chain, front view.
PDB entry 1xxx coloured by chain, side view.
PDB entry 1xxx coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure and kinetic study of dihydrodipicolinate synthase from Mycobacterium tuberculosis.
Kefala G, Evans GL, Griffin MD, Devenish SR, Pearce FG, Perugini MA, Gerrard JA, Weiss MS, Dobson RC
Biochem J 411 351-60 (2008)
PMID: 18062777

Function and Biology Details

Reaction catalysed: 
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-tetrahydrodipicolinate synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 303 amino acids
Theoretical weight: 31.12 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WP25 (Residues: 1-300; Coverage: 100%)
Gene names: MTV002.18c, Rv2753c, dapA
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand MG 8 x MG
Ligand CL 8 x CL
Ligand DTT 8 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 94.79Å b: 87.37Å c: 139.85Å
α: 90° β: 107.78° γ: 90°
R-values:
R R work R free
0.149 0.148 0.215
Expression system: Escherichia coli

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Citations

1 review citation

Structure and function of Mycobacterium tuberculosis meso-diaminopimelic acid (DAP) biosynthetic enzymes.
Usha et al. (2012)
33 other articles

9 mentions without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)
8 more