› 1xx4

X-ray diffraction
2.2Å resolution

Crystal Structure of Rat Mitochondrial 3,2-Enoyl-CoA

Released:
DOI: 10.2210/pdb1xx4/pdb
PDB entry 1xx4 coloured by chain, front view.
PDB entry 1xx4 coloured by chain, side view.
PDB entry 1xx4 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Domain swapping in the low-similarity isomerase/hydratase superfamily: the crystal structure of rat mitochondrial Delta3, Delta2-enoyl-CoA isomerase.
Hubbard PA, Yu W, Schulz H, Kim JJ
Protein Sci 14 1545-55 (2005)
PMID: 15883186

Function and Biology Details

Reaction catalysed: 
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150135 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA delta isomerase 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 29.36 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P23965 (Residues: 29-289; Coverage: 90%)
Gene names: Dci, Eci1
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 3 x PO4
Ligand ZN 3 x ZN
Ligand BEN 3 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300, RIGAKU RU200
Spacegroup: I4132
Unit cell:
a: 169.918Å b: 169.918Å c: 169.918Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.239 0.265
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Peroxisomal beta-oxidation--a metabolic pathway with multiple functions.
Poirier et al. (2006)
1 more

1 mention without citation

Domain swapping in the low-similarity isomerase/hydratase superfamily: the crystal structure of rat mitochondrial Delta3, Delta2-enoyl-CoA isomerase.
Hubbard et al. (2005)