› 1xw3

X-ray diffraction
1.65Å resolution

Crystal Structure of Human Sulfiredoxin (Srx)

Released:
DOI: 10.2210/pdb1xw3/pdb
PDB entry 1xw3 coloured by chain, front view.
PDB entry 1xw3 coloured by chain, side view.
PDB entry 1xw3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the retroreduction of inactivated peroxiredoxins by human sulfiredoxin.
Jönsson TJ, Murray MS, Johnson LC, Poole LB, Lowther WT
Biochemistry 44 8634-42 (2005)
PMID: 15952770

Function and Biology Details

Reaction catalysed: 
Peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH = peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189839 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfiredoxin-1 Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 12.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BYN0 (Residues: 32-137; Coverage: 77%)
Gene names: C20orf139, SRX, SRX1, SRXN1
Structure domains: Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P3221
Unit cell:
a: 68.057Å b: 68.057Å c: 51.302Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.214 0.258
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The basics of thiols and cysteines in redox biology and chemistry.
Poole LB. (2015)
7 more

10 mentions without citation

Reduction of cysteine sulfinic acid in eukaryotic, typical 2-Cys peroxiredoxins by sulfiredoxin.
Lowther et al. (2011)
9 more