› 1xql

X-ray diffraction
1.8Å resolution

Effect of a Y265F Mutant on the Transamination Based Cycloserine Inactivation of Alanine Racemase

Released:
DOI: 10.2210/pdb1xql/pdb
PDB entry 1xql coloured by chain, front view.
PDB entry 1xql coloured by chain, side view.
PDB entry 1xql coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Effect of a Y265F mutant on the transamination-based cycloserine inactivation of alanine racemase.
Fenn TD, Holyoak T, Stamper GF, Ringe D
Biochemistry 44 5317-27 (2005)
PMID: 15807525

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.68 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10724 (Residues: 1-388; Coverage: 100%)
Gene names: alr, dal
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 2 x PMP

Cofactor: Ligand PLP 2 x PLP
3 bound ligands:
Ligand PMH 2 x PMH
Ligand 4AX 2 x 4AX
Ligand ACY 2 x ACY
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 98.776Å b: 89.795Å c: 85.177Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.214
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
2 more

4 mentions without citation

The Recognition of Identical Ligands by Unrelated Proteins.
Barelier et al. (2015)
3 more