› 1xnf

X-ray diffraction
1.98Å resolution

Crystal structure of E.coli TPR-protein NlpI

Released:
DOI: 10.2210/pdb1xnf/pdb
PDB entry 1xnf coloured by chain, front view.
PDB entry 1xnf coloured by chain, side view.
PDB entry 1xnf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of NlpI. A prokaryotic tetratricopeptide repeat protein with a globular fold.
Wilson CG, Kajander T, Regan L
FEBS J 272 166-79 (2005)
PMID: 15634341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipoprotein NlpI Chains: A, B
Molecule details ›
Chains: A, B
Length: 275 amino acids
Theoretical weight: 31.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFB1 (Residues: 20-294; Coverage: 100%)
Gene names: JW3132, b3163, nlpI, yhbM
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

1 bound ligand:
Ligand TRS 2 x TRS
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 64.351Å b: 81.654Å c: 136.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

An Analytical Review of the Structural Features of Pentatricopeptide Repeats: Strategic Amino Acids, Repeat Arrangements and Superhelical Architecture.
Barik S. (2021)
22 other articles

6 mentions without citation

TPRpred: a tool for prediction of TPR-, PPR- and SEL1-like repeats from protein sequences.
Karpenahalli et al. (2007)
5 more