1xiz

X-ray diffraction
2Å resolution

Structural Genomics, The crystal structure of domain IIA of putative phosphotransferase system specific for mannitol/fructose from Salmonella typhimurium

Released:
Entry authors: Zhang R, Joachimiak G, Otwinowski Z, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-187245 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS EIIA type-2 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.02 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZL18 (Residues: 1-157; Coverage: 100%)
Gene name: STM3784
Sequence domains: Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2
Structure domains: Mannitol-specific EII; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 47.875Å b: 48.138Å c: 123.883Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.203 0.276
Expression system: Escherichia coli BL21(DE3)