› 1xhc

X-ray diffraction
2.35Å resolution

NADH oxidase /nitrite reductase from Pyrococcus furiosus Pfu-1140779-001

Released:
DOI: 10.2210/pdb1xhc/pdb
PDB entry 1xhc coloured by chain, front view.
PDB entry 1xhc coloured by chain, side view.
PDB entry 1xhc coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Entry authors: Horanyi P, Tempel W, Weinberg MV, Liu Z-J, Shah A, Chen L, Lee D, Sugar FJ, Brereton PS, Izumi M, Poole II FL, Shah C, Jenney Jr FE, Arendall III WB, Rose JP, Adams MWW, Richardson JS, Richardson DC, Wang B-C, Southeast Collaboratory for Structural Genomics (SECSG)

Function and Biology Details

Reaction catalysed: 
2 reduced rubredoxin + NAD(P)(+) + H(+) = 2 oxidized rubredoxin + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)H:rubredoxin oxidoreductase Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 40.91 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1K9 (Residues: 2-359; Coverage: 100%)
Gene name: PF1197
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 60.335Å b: 73.737Å c: 95.849Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.241
Expression system: Escherichia coli

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