1xfz Summary

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Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 1 millimolar exogenously added calcium chloride

The structure was published by Shen, Y., Zhukovskaya, N.L., Guo, Q., Florian, J., and Tang, W.J., in 2005 in a paper entitled "Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.25 Å and deposited in 2004.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Calmodulin-sensitive adenylate cyclase and Calmodulin 2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
B Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
C Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
D Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
E Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
F Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
O Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
P Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
Q Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
R Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
S Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
T Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P40136 (33 - 800) Calmodulin-sensitive adenylate cyclase Bacillus anthracis
P62158 (1 - 149) Calmodulin 2 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C, D, E, F (P40136) Collagenase (Catalytic Domain)search PF03497: Anthrax toxin LF subunitsearch, PF07737: Anthrax toxin lethal factor, N- and C-terminal domainsearch
O, P, Q, R, S, T (P62158) Calmodulin-likesearch EF-handsearch PF00036: EF handsearch, PF13499: EF-hand domain pairsearch, PF13833: EF-hand domain pairsearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A, B, C, D, E, F (P40136) metallopeptidase activitysearch calcium- and calmodulin-responsive adenylate cyclase activitysearch catalytic activitysearch metal ion bindingsearch extracellular regionsearch pathogenesissearch
O, P, Q, R, S, T (P62158) protein bindingsearch calcium ion bindingsearch thioesterase bindingsearch protein serine/threonine kinase activator activitysearch ion channel bindingsearch protein kinase bindingsearch phospholipase bindingsearch protein phosphatase activator activitysearch enzyme regulator activitysearch nitric-oxide synthase regulator activitysearch protein N-terminus bindingsearch N-terminal myristoylation domain bindingsearch adenylate cyclase bindingsearch protein domain specific bindingsearch titin bindingsearch calcium-dependent protein bindingsearch metal ion bindingsearch phosphatidylinositol 3-kinase bindingsearch type 3 metabotropic glutamate receptor bindingsearch nitric-oxide synthase bindingsearch spindle polesearch cytosolsearch cytoplasmsearch nucleussearch sarcomeresearch nucleoplasmsearch plasma membranesearch calcium channel complexsearch spindle microtubulesearch extracellular regionsearch extracellular vesicular exosomesearch vesiclesearch neuron projectionsearch spindlesearch centrosomesearch growth conesearch cytoskeletonsearch regulation of high voltage-gated calcium channel activitysearch regulation of cell communication by electrical coupling involved in cardiac conductionsearch positive regulation of nitric-oxide synthase activitysearch activation of adenylate cyclase activitysearch blood coagulationsearch carbohydrate metabolic processsearch glucose metabolic processsearch small molecule metabolic processsearch regulation of cytokinesissearch signal transductionsearch rhodopsin mediated signaling pathwaysearch membrane organizationsearch positive regulation of peptidyl-threonine phosphorylationsearch positive regulation of cyclic-nucleotide phosphodiesterase activitysearch synaptic transmissionsearch regulation of cardiac muscle contractionsearch substantia nigra developmentsearch platelet degranulationsearch positive regulation of protein serine/threonine kinase activitysearch positive regulation of protein autophosphorylationsearch nitric oxide metabolic processsearch calcium-mediated signalingsearch regulation of rhodopsin mediated signaling pathwaysearch Fc-epsilon receptor signaling pathwaysearch positive regulation of ryanodine-sensitive calcium-release channel activitysearch inositol phosphate metabolic processsearch muscle contractionsearch detection of calcium ionsearch positive regulation of phosphoprotein phosphatase activitysearch regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulumsearch fibroblast growth factor receptor signaling pathwaysearch neurotrophin TRK receptor signaling pathwaysearch platelet activationsearch response to calcium ionsearch regulation of nitric-oxide synthase activitysearch positive regulation of cyclic nucleotide metabolic processsearch activation of phospholipase C activitysearch negative regulation of peptidyl-threonine phosphorylationsearch positive regulation of protein dephosphorylationsearch G-protein coupled receptor signaling pathwaysearch regulation of ryanodine-sensitive calcium-release channel activitysearch innate immune responsesearch response to corticosteronesearch regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionsearch regulation of heart ratesearch phototransduction, visible lightsearch negative regulation of ryanodine-sensitive calcium-release channel activitysearch epidermal growth factor receptor signaling pathwaysearch glycogen catabolic processsearch response to amphetaminesearch

Chain InterPro annotation
A, B, C, D, E, F Anthrax toxin, lethal/endema factorsearch Anthrax toxin, edema factor, centralsearch Anthrax toxin, lethal/endema factor, N-/C-terminalsearch Metallopeptidase, catalytic domainsearch
O, P, Q, R, S, T EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch