1xfy Summary

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Crystal structure of anthrax edema factor (EF) in complex with calmodulin

The structure was published by Shen, Y., Zhukovskaya, N.L., Guo, Q., Florian, J., and Tang, W.J., in 2005 in a paper entitled "Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.3 Å and deposited in 2004.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Calmodulin-sensitive adenylate cyclase and Calmodulin 2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
B Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
C Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
D Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
E Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
F Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
O Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
P Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
Q Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
R Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
S Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
T Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P40136 (33 - 800) Calmodulin-sensitive adenylate cyclase Bacillus anthracis
P62158 (1 - 149) Calmodulin 2 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C, D, E, F (P40136) Collagenase (Catalytic Domain)search PF03497: Anthrax toxin LF subunitsearch, PF07737: Anthrax toxin lethal factor, N- and C-terminal domainsearch
O, P, Q, R, S, T (P62158) Calmodulin-likesearch EF-handsearch PF00036: EF handsearch, PF13499: EF-hand domain pairsearch, PF13833: EF-hand domain pairsearch

Chain ID Cellular component (GO) Biological process (GO) Molecular function (GO)
A, B, C, D, E, F (P40136) extracellular regionsearch pathogenesissearch metal ion bindingsearch catalytic activitysearch metallopeptidase activitysearch calcium- and calmodulin-responsive adenylate cyclase activitysearch
O, P, Q, R, S, T (P62158) nucleoplasmsearch cytosolsearch growth conesearch extracellular vesicular exosomesearch nucleussearch cytoskeletonsearch plasma membranesearch extracellular regionsearch cytoplasmsearch spindlesearch spindle polesearch vesiclesearch spindle microtubulesearch sarcomeresearch neuron projectionsearch centrosomesearch calcium channel complexsearch innate immune responsesearch activation of adenylate cyclase activitysearch regulation of cell communication by electrical coupling involved in cardiac conductionsearch regulation of ryanodine-sensitive calcium-release channel activitysearch positive regulation of protein dephosphorylationsearch regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulumsearch signal transductionsearch blood coagulationsearch activation of phospholipase C activitysearch detection of calcium ionsearch G-protein coupled receptor signaling pathwaysearch positive regulation of cyclic nucleotide metabolic processsearch positive regulation of peptidyl-threonine phosphorylationsearch synaptic transmissionsearch regulation of cardiac muscle contractionsearch nitric oxide metabolic processsearch platelet degranulationsearch positive regulation of cyclic-nucleotide phosphodiesterase activitysearch phototransduction, visible lightsearch glucose metabolic processsearch Fc-epsilon receptor signaling pathwaysearch regulation of nitric-oxide synthase activitysearch membrane organizationsearch response to corticosteronesearch positive regulation of ryanodine-sensitive calcium-release channel activitysearch negative regulation of ryanodine-sensitive calcium-release channel activitysearch substantia nigra developmentsearch regulation of rhodopsin mediated signaling pathwaysearch negative regulation of peptidyl-threonine phosphorylationsearch inositol phosphate metabolic processsearch platelet activationsearch regulation of cytokinesissearch positive regulation of phosphoprotein phosphatase activitysearch glycogen catabolic processsearch muscle contractionsearch small molecule metabolic processsearch positive regulation of protein autophosphorylationsearch rhodopsin mediated signaling pathwaysearch response to amphetaminesearch positive regulation of nitric-oxide synthase activitysearch regulation of heart ratesearch calcium-mediated signalingsearch fibroblast growth factor receptor signaling pathwaysearch epidermal growth factor receptor signaling pathwaysearch positive regulation of protein serine/threonine kinase activitysearch neurotrophin TRK receptor signaling pathwaysearch carbohydrate metabolic processsearch regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionsearch response to calcium ionsearch regulation of high voltage-gated calcium channel activitysearch metal ion bindingsearch protein bindingsearch calcium ion bindingsearch type 3 metabotropic glutamate receptor bindingsearch N-terminal myristoylation domain bindingsearch protein N-terminus bindingsearch nitric-oxide synthase bindingsearch protein phosphatase activator activitysearch phosphatidylinositol 3-kinase bindingsearch nitric-oxide synthase regulator activitysearch phospholipase bindingsearch adenylate cyclase bindingsearch calcium-dependent protein bindingsearch thioesterase bindingsearch protein domain specific bindingsearch protein kinase bindingsearch ion channel bindingsearch enzyme regulator activitysearch protein serine/threonine kinase activator activitysearch titin bindingsearch

Chain InterPro annotation
A, B, C, D, E, F Anthrax toxin, lethal/endema factorsearch Anthrax toxin, edema factor, centralsearch Anthrax toxin, lethal/endema factor, N-/C-terminalsearch Metallopeptidase, catalytic domainsearch
O, P, Q, R, S, T EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch