1xfx Summary

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Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 10 millimolar exogenously added calcium chloride

The structure was published by Shen, Y., Zhukovskaya, N.L., Guo, Q., Florian, J., and Tang, W.J., in 2005 in a paper entitled "Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.2 Å and deposited in 2004.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Calmodulin-sensitive adenylate cyclase and Calmodulin 2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
B Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
C Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
D Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
E Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
F Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
O Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
P Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
Q Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
R Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
S Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
T Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P40136 (33 - 800) Calmodulin-sensitive adenylate cyclase Bacillus anthracis
P62158 (1 - 149) Calmodulin 2 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C, D, E, F (P40136) Collagenase (Catalytic Domain)search PF03497: Anthrax toxin LF subunitsearch, PF07737: Anthrax toxin lethal factor, N- and C-terminal domainsearch
O, P, Q, R, S, T (P62158) Calmodulin-likesearch EF-handsearch PF00036: EF handsearch, PF13499: EF-hand domain pairsearch, PF13833: EF-hand domain pairsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B, C, D, E, F (P40136) metal ion bindingsearch metallopeptidase activitysearch calcium- and calmodulin-responsive adenylate cyclase activitysearch catalytic activitysearch pathogenesissearch extracellular regionsearch
O, P, Q, R, S, T (P62158) phospholipase bindingsearch protein bindingsearch calcium ion bindingsearch thioesterase bindingsearch phosphatidylinositol 3-kinase bindingsearch titin bindingsearch ion channel bindingsearch calcium-dependent protein bindingsearch protein domain specific bindingsearch metal ion bindingsearch protein phosphatase activator activitysearch N-terminal myristoylation domain bindingsearch synaptic transmissionsearch platelet activationsearch signal transductionsearch response to amphetaminesearch neurotrophin TRK receptor signaling pathwaysearch positive regulation of ryanodine-sensitive calcium-release channel activitysearch innate immune responsesearch Fc-epsilon receptor signaling pathwaysearch regulation of cardiac muscle contractionsearch rhodopsin mediated signaling pathwaysearch detection of calcium ionsearch fibroblast growth factor receptor signaling pathwaysearch positive regulation of protein dephosphorylationsearch regulation of cell communication by electrical coupling involved in cardiac conductionsearch muscle contractionsearch response to calcium ionsearch carbohydrate metabolic processsearch positive regulation of cyclic nucleotide metabolic processsearch regulation of cytokinesissearch inositol phosphate metabolic processsearch regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulumsearch phototransduction, visible lightsearch regulation of nitric-oxide synthase activitysearch epidermal growth factor receptor signaling pathwaysearch blood coagulationsearch positive regulation of phosphoprotein phosphatase activitysearch positive regulation of cyclic-nucleotide phosphodiesterase activitysearch response to corticosterone stimulussearch activation of phospholipase C activitysearch glucose metabolic processsearch negative regulation of ryanodine-sensitive calcium-release channel activitysearch small molecule metabolic processsearch G-protein coupled receptor signaling pathwaysearch regulation of rhodopsin mediated signaling pathwaysearch platelet degranulationsearch glycogen catabolic processsearch regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionsearch nitric oxide metabolic processsearch regulation of heart ratesearch cytosolsearch nucleoplasmsearch cytoplasmsearch centrosomesearch extracellular regionsearch plasma membranesearch spindle microtubulesearch spindlesearch spindle polesearch sarcomeresearch calcium channel complexsearch cytoskeletonsearch

Chain InterPro annotation
A, B, C, D, E, F Anthrax toxin, lethal/endema factorsearch Anthrax toxin, edema factor, centralsearch Anthrax toxin, lethal/endema factor, N-/C-terminalsearch Metallopeptidase, catalytic domainsearch
O, P, Q, R, S, T Recoverin likesearch EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch