1xfw Summary

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Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3'5' cyclic AMP (cAMP)

The structure was published by Shen, Y., Zhukovskaya, N.L., Guo, Q., Florian, J., and Tang, W.J., in 2005 in a paper entitled "Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.4 Å and deposited in 2004.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Calmodulin-sensitive adenylate cyclase and Calmodulin 2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
B Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
C Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
D Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
E Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
F Calmodulin-sensitive adenylate cyclase P40136 (33-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 777 94%
O Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
P Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
Q Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
R Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
S Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%
T Calmodulin 2 P62158 (1-149) (CALM_HUMAN)search Homo sapienssearch 97% 149 97%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P40136 (33 - 800) Calmodulin-sensitive adenylate cyclase Bacillus anthracis
P62158 (1 - 149) Calmodulin 2 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C, D, E, F (P40136) Collagenase (Catalytic Domain)search PF03497: Anthrax toxin LF subunitsearch, PF07737: Anthrax toxin lethal factor, N- and C-terminal domainsearch
O, P, Q, R, S, T (P62158) Calmodulin-likesearch EF-handsearch PF13499: EF-hand domain pairsearch, PF13833: EF-hand domain pairsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B, C, D, E, F (P40136) metallopeptidase activitysearch catalytic activitysearch calcium- and calmodulin-responsive adenylate cyclase activitysearch metal ion bindingsearch pathogenesissearch extracellular regionsearch
O, P, Q, R, S, T (P62158) calcium ion bindingsearch ion channel bindingsearch protein bindingsearch protein N-terminus bindingsearch N-terminal myristoylation domain bindingsearch enzyme regulator activitysearch titin bindingsearch type 3 metabotropic glutamate receptor bindingsearch calcium-dependent protein bindingsearch nitric-oxide synthase bindingsearch protein phosphatase activator activitysearch phospholipase bindingsearch protein domain specific bindingsearch phosphatidylinositol 3-kinase bindingsearch thioesterase bindingsearch metal ion bindingsearch adenylate cyclase bindingsearch nitric-oxide synthase regulator activitysearch synaptic transmissionsearch platelet degranulationsearch positive regulation of phosphoprotein phosphatase activitysearch glucose metabolic processsearch inositol phosphate metabolic processsearch regulation of heart ratesearch positive regulation of protein dephosphorylationsearch blood coagulationsearch small molecule metabolic processsearch regulation of cardiac muscle contractionsearch positive regulation of nitric-oxide synthase activitysearch platelet activationsearch Fc-epsilon receptor signaling pathwaysearch calcium-mediated signalingsearch fibroblast growth factor receptor signaling pathwaysearch positive regulation of cyclic nucleotide metabolic processsearch positive regulation of ryanodine-sensitive calcium-release channel activitysearch muscle contractionsearch activation of phospholipase C activitysearch regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulumsearch regulation of high voltage-gated calcium channel activitysearch phototransduction, visible lightsearch G-protein coupled receptor signaling pathwaysearch regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionsearch regulation of nitric-oxide synthase activitysearch carbohydrate metabolic processsearch regulation of cytokinesissearch response to calcium ionsearch regulation of cell communication by electrical coupling involved in cardiac conductionsearch nitric oxide metabolic processsearch membrane organizationsearch glycogen catabolic processsearch signal transductionsearch regulation of rhodopsin mediated signaling pathwaysearch response to corticosteronesearch detection of calcium ionsearch activation of adenylate cyclase activitysearch epidermal growth factor receptor signaling pathwaysearch positive regulation of cyclic-nucleotide phosphodiesterase activitysearch rhodopsin mediated signaling pathwaysearch regulation of ryanodine-sensitive calcium-release channel activitysearch response to amphetaminesearch substantia nigra developmentsearch negative regulation of ryanodine-sensitive calcium-release channel activitysearch innate immune responsesearch neurotrophin TRK receptor signaling pathwaysearch plasma membranesearch growth conesearch neuron projectionsearch spindle polesearch cytosolsearch nucleoplasmsearch calcium channel complexsearch nucleussearch extracellular regionsearch spindle microtubulesearch centrosomesearch cytoplasmsearch extracellular vesicular exosomesearch cytoskeletonsearch spindlesearch sarcomeresearch

Chain InterPro annotation
A, B, C, D, E, F Anthrax toxin, lethal/endema factorsearch Anthrax toxin, edema factor, centralsearch Anthrax toxin, lethal/endema factor, N-/C-terminalsearch Metallopeptidase, catalytic domainsearch
O, P, Q, R, S, T EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch