› 1xfp

X-ray diffraction
1.5Å resolution

Crystal structure of the CDR2 germline reversion mutant of cAb-Lys3 in complex with hen egg white lysozyme

Released:
DOI: 10.2210/pdb1xfp/pdb
PDB entry 1xfp coloured by chain, front view.
PDB entry 1xfp coloured by chain, side view.
PDB entry 1xfp coloured by chain, top view.
Source organisms:
Primary publication:
Chemical basis for the affinity maturation of a camel single domain antibody.
De Genst E, Handelberg F, Van Meirhaeghe A, Vynck S, Loris R, Wyns L, Muyldermans S
J Biol Chem 279 53593-601 (2004)
PMID: 15383540

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208249 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
heavy chain antibody Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 15.13 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Lysozyme C Chain: L
Molecule details ›
Chain: L
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand FMT 8 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2
Unit cell:
a: 128.294Å b: 72.334Å c: 38.766Å
α: 90° β: 106.74° γ: 90°
R-values:
R R work R free
0.202 0.201 0.216
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
10 more

8 mentions without citation

Electrostatic properties of protein-protein complexes.
Kundrotas et al. (2006)
7 more