› 1xfa

X-ray diffraction
3.1Å resolution

Structure of NBD1 from murine CFTR- F508R mutant

Released:
DOI: 10.2210/pdb1xfa/pdb
PDB entry 1xfa coloured by chain, front view.
PDB entry 1xfa coloured by chain, side view.
PDB entry 1xfa coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Side chain and backbone contributions of Phe508 to CFTR folding.
Thibodeau PH, Brautigam CA, Machius M, Thomas PJ
Nat Struct Mol Biol 12 10-6 (2005)
PMID: 15619636

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystic fibrosis transmembrane conductance regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 31.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P26361 (Residues: 389-670; Coverage: 19%)
Gene names: Abcc7, Cftr
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand CL 2 x CL
Ligand ATP 2 x ATP
Ligand ACY 4 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I4122
Unit cell:
a: 139.994Å b: 139.994Å c: 278.721Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.249 0.261
Expression system: Escherichia coli

Quick links

Citations

23 review citations

The ABC protein turned chloride channel whose failure causes cystic fibrosis.
Gadsby et al. (2006)
22 more

4 mentions without citation

Cystic fibrosis transmembrane conductance regulator (ABCC7) structure.
Hunt et al. (2013)
3 more