› 1xb4

X-ray diffraction
3.1Å resolution

Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II

Released:
DOI: 10.2210/pdb1xb4/pdb
PDB entry 1xb4 coloured by chain, front view.
PDB entry 1xb4 coloured by chain, side view.
PDB entry 1xb4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II.
Wernimont AK, Weissenhorn W
BMC Struct Biol 4 10 (2004)
PMID: 15579210

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein-sorting-associated protein 25 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 202 amino acids
Theoretical weight: 23.58 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P47142 (Residues: 1-202; Coverage: 100%)
Gene names: J1957, VPS25, VPT25, YJR102C
Sequence domains: ESCRT-II complex subunit
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 53.36Å b: 123.66Å c: 140.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.273 0.327 0.327
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The emerging shape of the ESCRT machinery.
Williams et al. (2007)
4 more

2 mentions without citation

Asgard archaea shed light on the evolutionary origins of the eukaryotic ubiquitin-ESCRT machinery.
Hatano et al. (2022)
1 more