› 1x9u

X-ray diffraction
1.8Å resolution

Umecyanin from Horse Raddish- Crystal Structure of the reduced form

Released:
DOI: 10.2210/pdb1x9u/pdb
PDB entry 1x9u coloured by chain, front view.
PDB entry 1x9u coloured by chain, side view.
PDB entry 1x9u coloured by chain, top view.
Source organism: Armoracia rusticana
Primary publication:
Crystal structures of oxidized and reduced stellacyanin from horseradish roots.
Koch M, Velarde M, Harrison MD, Echt S, Fischer M, Messerschmidt A, Dennison C
J Am Chem Soc 127 158-66 (2005)
PMID: 15631465

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154896 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Umecyanin Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 12.52 KDa
Source organism: Armoracia rusticana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42849 (Residues: 1-115; Coverage: 100%)
Sequence domains: Plastocyanin-like domain
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P65
Unit cell:
a: 92.65Å b: 92.65Å c: 47.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
4 more