› 1x7h

X-ray diffraction
2.3Å resolution

Actinorhodin Polyketide Ketoreductase, with NADPH bound

Released:
DOI: 10.2210/pdb1x7h/pdb
PDB entry 1x7h coloured by chain, front view.
PDB entry 1x7h coloured by chain, side view.
PDB entry 1x7h coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Structural analysis of actinorhodin polyketide ketoreductase: cofactor binding and substrate specificity.
Korman TP, Hill JA, Vu TN, Tsai SC
Biochemistry 43 14529-38 (2004)
PMID: 15544323

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative ketoacyl reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 27.29 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P16544 (Residues: 1-261; Coverage: 100%)
Gene names: SCBAC28G1.12c, SCO5086, actIII
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NDP 2 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3221
Unit cell:
a: 103.703Å b: 103.703Å c: 122.684Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.246
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Type II polyketide synthases: gaining a deeper insight into enzymatic teamwork.
Hertweck et al. (2007)
11 more

5 mentions without citation

Biosynthesis of aromatic polyketides in bacteria.
Das et al. (2009)
4 more