› 1wzn

X-ray diffraction
1.9Å resolution

Crystal Structure of the SAM-dependent methyltransferase from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb1wzn/pdb
PDB entry 1wzn coloured by chain, front view.
PDB entry 1wzn coloured by chain, side view.
PDB entry 1wzn coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129792 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 252 amino acids
Theoretical weight: 29.91 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59000 (Residues: 1-252; Coverage: 100%)
Gene name: PH1305
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand MG 1 x MG
Ligand SAH 3 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2221
Unit cell:
a: 108.357Å b: 132.011Å c: 145.991Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.233
Expression system: Escherichia coli

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