› 1wyk

X-ray diffraction
2Å resolution

SINDBIS VIRUS CAPSID PROTEIN (114-264)

Released:
DOI: 10.2210/pdb1wyk/pdb
PDB entry 1wyk coloured by chain, front view.
PDB entry 1wyk coloured by chain, side view.
PDB entry 1wyk coloured by chain, top view.
Source organism: Sindbis virus
Primary publication:
Probing the potential glycoprotein binding site of sindbis virus capsid protein with dioxane and model building.
Lee S, Kuhn RJ, Rossmann MG
Proteins 33 311-7 (1998)
PMID: 9779796

Function and Biology Details

Reaction catalysed: 
Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 16.68 KDa
Source organism: Sindbis virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27285 (Residues: 114-264; Coverage: 12%)
Sequence domains: Alphavirus core protein
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand FOR 4 x FOR
Ligand DIO 5 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 35.98Å b: 59.54Å c: 71.05Å
α: 109.4° β: 101.5° γ: 90.14°
R-values:
R R work R free
0.197 0.197 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Membrane fusion: a structural perspective on the interplay of lipids and proteins.
Tamm et al. (2003)
3 more

5 mentions without citation

Alphavirus Nucleocapsid Packaging and Assembly.
Mendes et al. (2018)
4 more