› 1wxr

X-ray diffraction
2.2Å resolution

Crystal structure of Heme Binding protein, an autotransporter hemoglobine protease from pathogenic Escherichia coli

Released:
DOI: 10.2210/pdb1wxr/pdb
PDB entry 1wxr coloured by chain, front view.
PDB entry 1wxr coloured by chain, side view.
PDB entry 1wxr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of hemoglobin protease, a heme binding autotransporter protein from pathogenic Escherichia coli.
Otto BR, Sijbrandi R, Luirink J, Oudega B, Heddle JG, Mizutani K, Park SY, Tame JR
J Biol Chem 280 17339-45 (2005)
PMID: 15728184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemoglobin-binding protease hbp Chain: A
Molecule details ›
Chain: A
Length: 1048 amino acids
Theoretical weight: 111.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: O88093 (Residues: 53-1100; Coverage: 79%)
Gene name: hbp
Sequence domains: Immunoglobulin A1 protease
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45PX, PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P6122
Unit cell:
a: 115.028Å b: 115.028Å c: 437.057Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.203 0.243
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Protein secretion in gram-negative bacteria via the autotransporter pathway.
Dautin et al. (2007)
26 more

28 mentions without citation

Serine protease autotransporters of enterobacteriaceae (SPATEs): biogenesis and function.
Dautin N. (2010)
27 more