› 1wx1

X-ray diffraction
1.97Å resolution

Crystal structure of nictinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1wx1/pdb
PDB entry 1wx1 coloured by chain, front view.
PDB entry 1wx1 coloured by chain, side view.
PDB entry 1wx1 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Asada Y, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 34.79 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7SIC7 (Residues: 1-335; Coverage: 100%)
Gene names: TTHB048, cobT
Sequence domains: Phosphoribosyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: I422
Unit cell:
a: 107.357Å b: 107.357Å c: 244.665Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.219
Expression system: Escherichia coli BL21(DE3)

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