› 1wp9

X-ray diffraction
2.9Å resolution

Crystal structure of Pyrococcus furiosus Hef helicase domain

Released:
DOI: 10.2210/pdb1wp9/pdb
PDB entry 1wp9 coloured by chain, front view.
PDB entry 1wp9 coloured by chain, side view.
PDB entry 1wp9 coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Crystal structure and functional implications of Pyrococcus furiosus hef helicase domain involved in branched DNA processing.
Nishino T, Komori K, Tsuchiya D, Ishino Y, Morikawa K
Structure 13 143-53 (2005)
PMID: 15642269

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186323 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase, putative Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 494 amino acids
Theoretical weight: 56.34 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TZH8 (Residues: 2-495; Coverage: 65%)
Gene name: PF2015
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 118.498Å b: 165.94Å c: 119.157Å
α: 90° β: 114.85° γ: 90°
R-values:
R R work R free
0.286 0.257 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

SF1 and SF2 helicases: family matters.
Fairman-Williams et al. (2010)
14 more

13 mentions without citation

Mechanisms for ATP-dependent chromatin remodelling: the means to the end.
Flaus et al. (2011)
12 more