› 1wp6

X-ray diffraction
2.1Å resolution

Crystal structure of maltohexaose-producing amylase from alkalophilic Bacillus sp.707.

Released:
DOI: 10.2210/pdb1wp6/pdb
PDB entry 1wp6 coloured by chain, front view.
PDB entry 1wp6 coloured by chain, side view.
PDB entry 1wp6 coloured by chain, top view.
Source organism: Bacillus sp. 707
Primary publication:
Biochemical and crystallographic analyses of maltohexaose-producing amylase from alkalophilic Bacillus sp. 707.
Kanai R, Haga K, Akiba T, Yamane K, Harata K
Biochemistry 43 14047-56 (2004)
PMID: 15518553

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in amylaceous polysaccharides, to remove successive maltohexaose residues from the non-reducing chain ends
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucan 1,4-alpha-maltohexaosidase Chain: A
Molecule details ›
Chain: A
Length: 485 amino acids
Theoretical weight: 55.44 KDa
Source organism: Bacillus sp. 707
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P19571 (Residues: 34-518; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 3 x CA
Ligand NA 1 x NA
Ligand PO4 1 x PO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 47.62Å b: 82.8Å c: 127.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 0.21
Expression system: Bacillus subtilis

Quick links

Citations

5 review citations

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases.
Janeček et al. (2014)
4 more

5 mentions without citation

Molecular characterization of α-amylase from Staphylococcus aureus.
Lakshmi et al. (2013)
4 more