› 1wkh

X-ray diffraction
2.25Å resolution

Acetylornithine aminotransferase from thermus thermophilus HB8

Released:
DOI: 10.2210/pdb1wkh/pdb
PDB entry 1wkh coloured by chain, front view.
PDB entry 1wkh coloured by chain, side view.
PDB entry 1wkh coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Matsumura M, Goto M, Omi R, Miyahara I, Hirotsu K, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
An [amino group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + 2-oxoglutarate = an [amino group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
[LysW]-aminoadipate semialdehyde transaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 395 amino acids
Theoretical weight: 43.51 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SHH5 (Residues: 1-395; Coverage: 100%)
Gene names: TTHA1755, lysJ
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PPE 2 x PPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 70.85Å b: 69.87Å c: 140.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.243
Expression system: Escherichia coli

Quick links