› 1wj9

X-ray diffraction
1.9Å resolution

Crystal structure of a CRISPR-associated protein from thermus thermophilus

Released:
DOI: 10.2210/pdb1wj9/pdb
PDB entry 1wj9 coloured by chain, front view.
PDB entry 1wj9 coloured by chain, side view.
PDB entry 1wj9 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structure of hypothetical protein TTHB192 from Thermus thermophilus HB8 reveals a new protein family with an RNA recognition motif-like domain.
Ebihara A, Yao M, Masui R, Tanaka I, Yokoyama S, Kuramitsu S
Protein Sci 15 1494-9 (2006)
PMID: 16672237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endoribonuclease Cse3 Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 23.76 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53WG9 (Residues: 1-211; Coverage: 100%)
Gene names: TTHB192, cas6, cse3
Sequence domains: CRISPR associated protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P65
Unit cell:
a: 76.673Å b: 76.673Å c: 87.494Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.228 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

RNA-guided genetic silencing systems in bacteria and archaea.
Wiedenheft et al. (2012)
19 more

11 mentions without citation

Evolution and classification of the CRISPR-Cas systems.
Makarova et al. (2011)
10 more