› 1wgi

X-ray diffraction
2.2Å resolution

STRUCTURE OF INORGANIC PYROPHOSPHATASE

Released:
DOI: 10.2210/pdb1wgi/pdb
PDB entry 1wgi coloured by chain, front view.
PDB entry 1wgi coloured by chain, side view.
PDB entry 1wgi coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The structural basis for pyrophosphatase catalysis.
Heikinheimo P, Lehtonen J, Baykov A, Lahti R, Cooperman BS, Goldman A
Structure 4 1491-508 (1996)
PMID: 8994974

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.23 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00817 (Residues: 2-287; Coverage: 100%)
Gene names: IPP1, PPA, PPA1, YBR011C, YBR0202
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 54.3Å b: 68.4Å c: 161.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.211
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Biological phosphoryl-transfer reactions: understanding mechanism and catalysis.
Lassila et al. (2011)
4 more

3 mentions without citation

Prediction of catalytic residues using Support Vector Machine with selected protein sequence and structural properties.
Petrova et al. (2006)
2 more