› 1wcs

X-ray diffraction
2.8Å resolution

A mutant of Trypanosoma rangeli sialidase displaying trans-sialidase activity

Released:
DOI: 10.2210/pdb1wcs/pdb
PDB entry 1wcs coloured by chain, front view.
PDB entry 1wcs coloured by chain, side view.
PDB entry 1wcs coloured by chain, top view.
Source organism: Trypanosoma rangeli
Primary publication:
A sialidase mutant displaying trans-sialidase activity.
Paris G, Ratier L, Amaya MF, Nguyen T, Alzari PM, Frasch AC
J Mol Biol 345 923-34 (2005)
PMID: 15588836

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 641 amino acids
Theoretical weight: 70.07 KDa
Source organism: Trypanosoma rangeli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O44049 (Residues: 20-660; Coverage: 100%)
Sequence domains: BNR repeat-like domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 94.691Å b: 94.691Å c: 156.335Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.244 0.336
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Glycosidases: a key to tailored carbohydrates.
Bojarová et al. (2009)
10 more

3 mentions without citation

Rational design of a new Trypanosoma rangeli trans-sialidase for efficient sialylation of glycans.
Jers et al. (2014)
2 more