› 1wae

X-ray diffraction
1.95Å resolution

Crystal structure of H129V Mutant of Alcaligenes Xylosoxidans Nitrite Reductase

Released:
DOI: 10.2210/pdb1wae/pdb
PDB entry 1wae coloured by chain, front view.
PDB entry 1wae coloured by chain, side view.
PDB entry 1wae coloured by chain, top view.
Source organism: Achromobacter xylosoxidans
Primary publication:
Observation of an unprecedented Cu Bis-His site: crystal structure of the H129V mutant of nitrite reductase.
Ellis MJ, Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS
Inorg Chem 43 7591-3 (2004)
PMID: 15554622

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 36.53 KDa
Source organism: Achromobacter xylosoxidans
Expression system: Escherichia coli
UniProt:
  • Canonical: O68601 (Residues: 25-360; Coverage: 100%)
Gene names: O9570_16215, nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: R3
Unit cell:
a: 91.206Å b: 91.206Å c: 145.866Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.201 0.226
Expression system: Escherichia coli

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Citations

2 review citations

Binding and activation of nitrite and nitric oxide by copper nitrite reductase and corresponding model complexes.
Merkle et al. (2012)
1 more