› 1w99

X-ray diffraction
1.75Å resolution

Mosquito-larvicidal toxin Cry4Ba from Bacillus thuringiensis ssp. Israelensis

Released:
DOI: 10.2210/pdb1w99/pdb
PDB entry 1w99 coloured by chain, front view.
PDB entry 1w99 coloured by chain, side view.
PDB entry 1w99 coloured by chain, top view.
Source organism: Bacillus thuringiensis serovar israelensis
Primary publication:
Crystal structure of the mosquito-larvicidal toxin Cry4Ba and its biological implications.
Boonserm P, Davis P, Ellar DJ, Li J
J Mol Biol 348 363-82 (2005)
PMID: 15811374

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pesticidal crystal protein Cry4Ba Chain: A
Molecule details ›
Chain: A
Length: 558 amino acids
Theoretical weight: 63.26 KDa
Source organism: Bacillus thuringiensis serovar israelensis
Expression system: Bacillus thuringiensis serovar israelensis
UniProt:
  • Canonical: P05519 (Residues: 84-641; Coverage: 49%)
Gene names: bt8, cry4Ba, cryD2, cryIVB(a), isrH3
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BR 25 x BR
Ligand P6G 1 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: R32
Unit cell:
a: 185.8Å b: 185.8Å c: 187.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.205 0.218
Expression system: Bacillus thuringiensis serovar israelensis

Quick links

Citations

21 review citations

Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control.
Bravo et al. (2007)
20 more

19 mentions without citation

Role of receptors in Bacillus thuringiensis crystal toxin activity.
Pigott et al. (2007)
18 more