› 1w5t

X-ray diffraction
2.4Å resolution

Structure of the Aeropyrum Pernix ORC2 protein (ADPNP-ADP complexes)

Released:
DOI: 10.2210/pdb1w5t/pdb
PDB entry 1w5t coloured by chain, front view.
PDB entry 1w5t coloured by chain, side view.
PDB entry 1w5t coloured by chain, top view.
Source organism: Aeropyrum pernix
Primary publication:
Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix.
Singleton MR, Morales R, Grainge I, Cook N, Isupov MN, Wigley DB
J Mol Biol 343 547-57 (2004)
PMID: 15465044

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ORC1-type DNA replication protein 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 412 amino acids
Theoretical weight: 46.3 KDa
Source organism: Aeropyrum pernix
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9YFU8 (Residues: 1-410; Coverage: 100%)
Gene names: APE_0152.1, orc2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 3 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P31
Unit cell:
a: 61.489Å b: 61.489Å c: 267.865Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.211 0.279
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Evolutionary relationships and structural mechanisms of AAA+ proteins.
Erzberger et al. (2006)
14 more

6 mentions without citation

Crystal structure of the eukaryotic origin recognition complex.
Bleichert et al. (2015)
5 more