› 1vwl

X-ray diffraction
1.45Å resolution

STREPTAVIDIN-CYCLO-[5-S-VALERAMIDE-HPQGPPC]K-NH2, PH 3.5, I222 COMPLEX

Released:
DOI: 10.2210/pdb1vwl/pdb
PDB entry 1vwl coloured by chain, front view.
PDB entry 1vwl coloured by chain, side view.
PDB entry 1vwl coloured by chain, top view.
Source organisms:
Primary publication:
In crystals of complexes of streptavidin with peptide ligands containing the HPQ sequence the pKa of the peptide histidine is less than 3.0.
Katz BA, Cass RT
J Biol Chem 272 13220-8 (1997)
PMID: 9148939

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149720 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptavidin Chains: B, D
Molecule details ›
Chains: B, D
Length: 123 amino acids
Theoretical weight: 12.97 KDa
Source organism: Streptomyces avidinii
UniProt:
  • Canonical: P22629 (Residues: 37-159; Coverage: 77%)
Sequence domains: Avidin family
Structure domains: Avidin-like
PEPTIDE LIGAND CONTAINING HPQ Chains: M, P
Molecule details ›
Chains: M, P
Length: 9 amino acids
Theoretical weight: 863 Da
Source organism: Bothrops insularis

Ligands and Environments

1 bound ligand:
Ligand LEA 2 x LEA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 94.81Å b: 105.42Å c: 47.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.244

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Citations

1 review citation

Streptavidin-binding and -dimerizing ligands discovered by phage display, topochemistry, and structure-based design.
Katz BA. (1999)
13 other articles