› 1vs1

X-ray diffraction
2.3Å resolution

Crystal structure of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHP synthase) from Aeropyrum pernix in complex with Mn2+ and PEP

Released:
DOI: 10.2210/pdb1vs1/pdb
PDB entry 1vs1 coloured by chain, front view.
PDB entry 1vs1 coloured by chain, side view.
PDB entry 1vs1 coloured by chain, top view.
Source organism: Aeropyrum pernix
Entry authors: Shumilin IA, Zhou L, Wu J, Woodard RW, Bauerle R, Kretsinger RH

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195489 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DAHP synthetase I/KDSA domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 276 amino acids
Theoretical weight: 30.04 KDa
Source organism: Aeropyrum pernix
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9YEJ7 (Residues: 1-270; Coverage: 100%)
Gene names: APE_0581.1, aroG
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand PEP 4 x PEP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 54.768Å b: 130.404Å c: 78.362Å
α: 90° β: 110.61° γ: 90°
R-values:
R R work R free
0.176 0.173 0.218
Expression system: Escherichia coli BL21

Quick links