› 1vra

X-ray diffraction
2Å resolution

Crystal structure of Arginine biosynthesis bifunctional protein argJ (10175521) from Bacillus halodurans at 2.00 A resolution

Released:
DOI: 10.2210/pdb1vra/pdb
PDB entry 1vra coloured by chain, front view.
PDB entry 1vra coloured by chain, side view.
PDB entry 1vra coloured by chain, top view.
Source organism: Halalkalibacterium halodurans
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate
N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-191784 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Arginine biosynthesis bifunctional protein ArgJ alpha chain Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 22.35 KDa
Source organism: Halalkalibacterium halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K8V3 (Residues: 1-196; Coverage: 48%)
Gene names: BH2899, argJ
Sequence domains: ArgJ family
Structure domains: L-amino peptidase D-ALA esterase/amidase
Arginine biosynthesis bifunctional protein ArgJ beta chain Chain: B
Molecule details ›
Chain: B
Length: 215 amino acids
Theoretical weight: 23.2 KDa
Source organism: Halalkalibacterium halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K8V3 (Residues: 197-411; Coverage: 52%)
Gene names: BH2899, argJ
Sequence domains: ArgJ family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand UNL 1 x UNL
Ligand EDO 16 x EDO
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 70.658Å b: 70.658Å c: 222.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.14 0.158
Expression system: Escherichia coli

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