› 1vm9

X-ray diffraction
1.48Å resolution

The X-ray Structure of the cys84ala cys85ala double mutant of the [2Fe-2S] Ferredoxin subunit of Toluene-4-Monooxygenase from Pseudomonas Mendocina KR1

Released:
DOI: 10.2210/pdb1vm9/pdb
PDB entry 1vm9 coloured by chain, front view.
PDB entry 1vm9 coloured by chain, side view.
PDB entry 1vm9 coloured by chain, top view.
Source organism: Pseudomonas mendocina
Primary publication:
Structure of T4moC, the Rieske-type ferredoxin component of toluene 4-monooxygenase.
Moe LA, Bingman CA, Wesenberg GE, Phillips GN, Fox BG
Acta Crystallogr D Biol Crystallogr 62 476-82 (2006)
PMID: 16627939

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toluene-4-monooxygenase system, ferredoxin component Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 12.14 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00458 (Residues: 2-112; Coverage: 99%)
Gene name: tmoC
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand FES 1 x FES
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID, RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 44.51Å b: 52.4Å c: 83.556Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.176
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Microbial enzymes for aromatic compound hydroxylation.
Di Gennaro et al. (2011)
7 other articles

4 mentions without citation

Performance of ZDOCK in CAPRI rounds 20-26.
Vreven et al. (2013)
3 more