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A Triclinic Crystal Form of the Lectin Concanavalin A

Kanellopoulos PN, Tucker PA, Pavlou K, Agianian B, Hamodrakas SJ
J Struct Biol 117 16-23 (1996)
Cited: 7 times
EuropePMC logo PMID: 8812975

Abstract

The molecular structure of a triclinic crystal form of concanavalin A has been refined at 2.4 A resolution. The crystals have unit cell dimensions a = 78.8 A, b = 79.3 A, c = 133.3 A, alpha = 97.1degrees, beta = 90.2degrees, and gamma = 97.5degrees and contain two tetramers per asymmetric unit each with approximate 222 symmetry. The final crystallographic R-factor is 0.205 and the free-R-factor is 0.265 in the resolution range 6.0 to 2.4 A. The conformation of the tetramer is more similar to that found in concanavalin A saccharide complexes than in the previously reported I222 crystal form of uncomplexed concanavalin A. A comparison of the molecular packing between the two crystal forms shows a more open arrangement with large solvent channels through the crystal.

Articles citing this publication (7)

  1. Force spectroscopy of the double-tethered concanavalin-A mannose bond. Ratto TV, Langry KC, Rudd RE, Balhorn RL, Allen MJ, McElfresh MW. Biophys J 86 2430-2437 (2004)
  2. The crystal structure of the complex of concanavalin A with 4'-methylumbelliferyl-alpha-D-glucopyranoside. Hamodrakas SJ, Kanellopoulos PN, Pavlou K, Tucker PA. J Struct Biol 118 23-30 (1997)
  3. Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds. Grangeiro TB, Schriefer A, Calvete JJ, Raida M, Urbanke C, Barral-Netto M, Cavada BS. Eur J Biochem 248 43-48 (1997)
  4. SPR studies of carbohydrate-lectin interactions as useful tool for screening on lectin sources. Vornholt W, Hartmann M, Keusgen M. Biosens Bioelectron 22 2983-2988 (2007)
  5. Structural and biochemical analyses of concanavalin A circular permutation by jack bean asparaginyl endopeptidase. Nonis SG, Haywood J, Schmidberger JW, Mackie ERR, Soares da Costa TP, Bond CS, Mylne JS. Plant Cell 33 2794-2811 (2021)
  6. Innovative analytical system for screening on lectins. Hartmann M, Nikitin P, Keusgen M. Biosens Bioelectron 22 28-34 (2006)
  7. New crystal forms of Diocleinae lectins in the presence of different dimannosides. Moreno FB, Bezerra GA, de Oliveira TM, de Souza EP, da Rocha BA, Benevides RG, Delatorre P, Cavada BS, de Azevedo WF. Acta Crystallogr Sect F Struct Biol Cryst Commun 62 1100-1103 (2006)


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