› 1v3v

X-ray diffraction
2Å resolution

Crystal structure of leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase complexed with NADP and 15-oxo-PGE2

Released:
DOI: 10.2210/pdb1v3v/pdb
PDB entry 1v3v coloured by chain, front view.
PDB entry 1v3v coloured by chain, side view.
PDB entry 1v3v coloured by chain, top view.
Source organism: Cavia porcellus
Primary publication:
Structural basis of leukotriene B4 12-hydroxydehydrogenase/15-Oxo-prostaglandin 13-reductase catalytic mechanism and a possible Src homology 3 domain binding loop.
Hori T, Yokomizo T, Ago H, Sugahara M, Ueno G, Yamamoto M, Kumasaka T, Shimizu T, Miyano M
J Biol Chem 279 22615-23 (2004)
PMID: 15007077

Function and Biology Details

Reactions catalysed: 
11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)(+) = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H
A n-alkanal + NAD(P)(+) = an alk-2-enal + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prostaglandin reductase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 333 amino acids
Theoretical weight: 36.23 KDa
Source organism: Cavia porcellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9EQZ5 (Residues: 1-329; Coverage: 100%)
Gene names: Ltb4dh, Ptgr1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
2 bound ligands:
Ligand CL 6 x CL
Ligand 5OP 2 x 5OP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P21
Unit cell:
a: 58.553Å b: 77.46Å c: 79.095Å
α: 90° β: 102.29° γ: 90°
R-values:
R R work R free
0.194 0.194 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Thematic Review Series: Proteomics. An integrated omics analysis of eicosanoid biology.
Buczynski et al. (2009)
5 more

1 mention without citation

A Fifth of the Protein World: Rossmann-like Proteins as an Evolutionarily Successful Structural unit.
Medvedev et al. (2021)