› 1v3q

X-ray diffraction
2.8Å resolution

Structure of human PNP complexed with DDI

Released:
DOI: 10.2210/pdb1v3q/pdb
PDB entry 1v3q coloured by chain, front view.
PDB entry 1v3q coloured by chain, side view.
PDB entry 1v3q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of human purine nucleoside phosphorylase complexed with inosine and ddI.
Canduri F, dos Santos DM, Silva RG, Mendes MA, Basso LA, Palma MS, de Azevedo WF, Santos DS
Biochem Biophys Res Commun 313 907-14 (2004)
PMID: 14706628

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: E
Molecule details ›
Chain: E
Length: 288 amino acids
Theoretical weight: 32.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00491 (Residues: 2-289; Coverage: 100%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand 2DI 1 x 2DI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: R32
Unit cell:
a: 141.33Å b: 141.33Å c: 161.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.3 0.214 0.306
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Tumor lysis syndrome: new challenges and recent advances.
Wilson et al. (2014)
1 more

2 mentions without citation

Four generations of transition-state analogues for human purine nucleoside phosphorylase.
Ho et al. (2010)
1 more