› 1v2e

X-ray diffraction
2.6Å resolution

Crystal Structure of T.th HB8 Glutamine Aminotransferase complex with a-keto-g-methylthiobutyrate

Released:
DOI: 10.2210/pdb1v2e/pdb
PDB entry 1v2e coloured by chain, front view.
PDB entry 1v2e coloured by chain, side view.
PDB entry 1v2e coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structures of glutamine:phenylpyruvate aminotransferase from Thermus thermophilus HB8: induced fit and substrate recognition.
Goto M, Omi R, Miyahara I, Hosono A, Mizuguchi H, Hayashi H, Kagamiyama H, Hirotsu K
J Biol Chem 279 16518-25 (2004)
PMID: 14761974

Function and Biology Details

Reaction catalysed: 
L-glutamine + pyruvate = 2-oxoglutaramate + L-alanine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class I/classII domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 381 amino acids
Theoretical weight: 42.32 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WK2 (Residues: 1-381; Coverage: 100%)
Gene name: TthHB5018_10190
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand KMT 2 x KMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P3121
Unit cell:
a: 123.14Å b: 123.14Å c: 122.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.231 0.286
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, expression, and function of kynurenine aminotransferases in human and rodent brains.
Han et al. (2010)
3 more