› 1uy0

X-ray diffraction
1.65Å resolution

Carbohydrate binding module (CBM6cm-2) from Cellvibrio mixtus lichenase 5A in complex with glc-1,3-glc-1,4-glc-1,3-glc

Released:
DOI: 10.2210/pdb1uy0/pdb
PDB entry 1uy0 coloured by chain, front view.
PDB entry 1uy0 coloured by chain, side view.
PDB entry 1uy0 coloured by chain, top view.
Source organism: Cellvibrio mixtus
Primary publication:
The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5a in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities.
Pires VM, Henshaw JL, Prates JA, Bolam DN, Ferreira LM, Fontes CM, Henrissat B, Planas A, Gilbert HJ, Czjzek M
J Biol Chem 279 21560-8 (2004)
PMID: 15010454

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
CBM6 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 13.77 KDa
Source organism: Cellvibrio mixtus
Expression system: Escherichia coli
UniProt:
  • Canonical: O07653 (Residues: 493-622; Coverage: 22%)
Gene name: celB
Sequence domains: Carbohydrate binding module (family 6)
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
4 bound ligands:
Ligand CA 4 x CA
Ligand GOL 3 x GOL
Ligand CL 3 x CL
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 63.55Å b: 67.65Å c: 85.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.177
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
5 more

1 mention without citation

The C-terminal domain of the Arabinosyltransferase Mycobacterium tuberculosis EmbC is a lectin-like carbohydrate binding module.
Alderwick et al. (2011)