› 1ux7

X-ray diffraction
1.5Å resolution

Carbohydrate-Binding Module CBM36 in complex with calcium and xylotriose

Released:
DOI: 10.2210/pdb1ux7/pdb
PDB entry 1ux7 coloured by chain, front view.
PDB entry 1ux7 coloured by chain, side view.
PDB entry 1ux7 coloured by chain, top view.
Source organism: Paenibacillus polymyxa
Primary publication:
Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules.
Jamal-Talabani S, Boraston AB, Turkenburg JP, Tarbouriech N, Ducros VM, Davies GJ
Structure 12 1177-87 (2004)
PMID: 15242594

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Arabinoxylan arabinofuranohydrolase Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 12.68 KDa
Source organism: Paenibacillus polymyxa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45796 (Residues: 516-635; Coverage: 20%)
Gene name: xynD
Sequence domains: Carbohydrate binding module (family 6)
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 38.581Å b: 52.132Å c: 54.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
9 more

10 mentions without citation

Sculpting therapeutic monoclonal antibody N-glycans using endoglycosidases.
Trastoy et al. (2022)
9 more