› 1upx

X-ray diffraction
1.25Å resolution

The crystal structure of the Hybrid Cluster Protein from Desulfovibrio desulfuricans containing molecules in the oxidized and reduced states.

Released:
DOI: 10.2210/pdb1upx/pdb
PDB entry 1upx coloured by chain, front view.
PDB entry 1upx coloured by chain, side view.
PDB entry 1upx coloured by chain, top view.
Source organism: Desulfovibrio desulfuricans
Primary publication:
Structure of the hybrid cluster protein (HCP) from Desulfovibrio desulfuricans ATCC 27774 containing molecules in the oxidized and reduced states.
Macedo S, Aragão D, Mitchell EP, Lindley P
Acta Crystallogr D Biol Crystallogr 59 2065-71 (2003)
PMID: 14646063

Function and Biology Details

Reaction catalysed: 
NH(3) + H(2)O + acceptor = hydroxylamine + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxylamine reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 544 amino acids
Theoretical weight: 58.6 KDa
Source organism: Desulfovibrio desulfuricans
UniProt:
  • Canonical: Q01770 (Residues: 2-545; Coverage: 100%)
Gene names: Ddes_1829, hcp
Sequence domains: Prismane/CO dehydrogenase family
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SF4 2 x SF4
Ligand SF3 2 x SF3
Ligand FSO 2 x FSO
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P1
Unit cell:
a: 57.3Å b: 61.2Å c: 72Å
α: 82.8° β: 73.7° γ: 87.3°
R-values:
R R work R free
0.14 0.14 0.153

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Citations

4 citation in other articles

Nitric oxide stress resistance in Porphyromonas gingivalis is mediated by a putative hydroxylamine reductase.
Boutrin et al. (2012)
3 more

1 mention without citation

Structural and biochemical elucidation of class I hybrid cluster protein natively extracted from a marine methanogenic archaeon.
Lemaire et al. (2023)