› 1ui8

X-ray diffraction
1.8Å resolution

Site-directed mutagenesis of His592 involved in binding of copper ion in Arthrobacter globiformis amine oxidase

Released:
DOI: 10.2210/pdb1ui8/pdb
PDB entry 1ui8 coloured by chain, front view.
PDB entry 1ui8 coloured by chain, side view.
PDB entry 1ui8 coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Chemical rescue of a site-specific mutant of bacterial copper amine oxidase for generation of the topa quinone cofactor.
Matsunami H, Okajima T, Hirota S, Yamaguchi H, Hori H, Kuroda S, Tanizawa K
Biochemistry 43 2178-87 (2004)
PMID: 14979714

Function and Biology Details

Reaction catalysed: 
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylethylamine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 638 amino acids
Theoretical weight: 70.69 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P46881 (Residues: 1-638; Coverage: 100%)
Sequence domains: Copper amine oxidase, enzyme domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
1 modified residue:
Ligand TPQ 2 x TPQ

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Unit cell:
a: 157.762Å b: 63.21Å c: 184.244Å
α: 90° β: 111.64° γ: 90°
R-values:
R R work R free
0.218 0.217 0.258
Expression system: Escherichia coli

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Citations

4 citation in other articles

Genetic analyses and molecular characterization of the pathways involved in the conversion of 2-phenylethylamine and 2-phenylethanol into phenylacetic acid in Pseudomonas putida U.
Arias et al. (2008)
3 more

1 mention without citation

Copper active sites in biology.
Solomon et al. (2014)