› 1uf7

X-ray diffraction
1.9Å resolution

Crystal structure of C171A/V236A Mutant of N-carbamyl-D-amino acid amidohydrolase complexed with N-carbamyl-D-valine

Released:
DOI: 10.2210/pdb1uf7/pdb
PDB entry 1uf7 coloured by chain, front view.
PDB entry 1uf7 coloured by chain, side view.
PDB entry 1uf7 coloured by chain, top view.
Source organism: Agrobacterium sp.
Entry authors: Hashimoto H, Aoki M, Shimizu T, Nakai T, Morikawa H, Ikenaka Y, Takahashi S, Sato M

Function and Biology Details

Reaction catalysed: 
N-carbamoyl-D-amino acid + H(2)O = D-amino acid + NH(3) + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-carbamoyl-D-amino acid hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 34.14 KDa
Source organism: Agrobacterium sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P60327 (Residues: 2-304; Coverage: 100%)
Sequence domains: Carbon-nitrogen hydrolase
Structure domains: Carbon-nitrogen hydrolase

Ligands and Environments

1 bound ligand:
Ligand CDV 2 x CDV
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 67.452Å b: 137.696Å c: 68.024Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.218
Expression system: Escherichia coli

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