› 1udz

X-ray diffraction
1.8Å resolution

Isoleucyl-tRNA synthetase editing domain

Released:
DOI: 10.2210/pdb1udz/pdb
PDB entry 1udz coloured by chain, front view.
PDB entry 1udz coloured by chain, side view.
PDB entry 1udz coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structures of the CP1 domain from Thermus thermophilus isoleucyl-tRNA synthetase and its complex with L-valine.
Fukunaga R, Fukai S, Ishitani R, Nureki O, Yokoyama S
J Biol Chem 279 8396-402 (2004)
PMID: 14672940

Function and Biology Details

Reaction catalysed: 
ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isoleucine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.16 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56690 (Residues: 200-381; Coverage: 17%)
Gene names: TTHA1067, ileS
Structure domains: Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P41212
Unit cell:
a: 102.739Å b: 102.739Å c: 83.834Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.25
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Transfer RNA: a dancer between charging and mis-charging for protein biosynthesis.
Zhou et al. (2013)
1 more

1 mention without citation

Coordination of tRNA synthetase active sites for chemical fidelity.
Boniecki et al. (2012)