› 1ua4

X-ray diffraction
1.9Å resolution

Crystal Structure of an ADP-dependent Glucokinase from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb1ua4/pdb
PDB entry 1ua4 coloured by chain, front view.
PDB entry 1ua4 coloured by chain, side view.
PDB entry 1ua4 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Crystal structure of an ADP-dependent glucokinase from Pyrococcus furiosus: implications for a sugar-induced conformational change in ADP-dependent kinase.
Ito S, Fushinobu S, Jeong JJ, Yoshioka I, Koga S, Shoun H, Wakagi T
J Mol Biol 331 871-83 (2003)
PMID: 12909015

Function and Biology Details

Reaction catalysed: 
ADP + D-glucosamine = AMP + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194188 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-dependent glucose/glucosamine kinase Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 51.33 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9V2Z6 (Residues: 1-455; Coverage: 100%)
Gene names: PF0312, glkA
Sequence domains: ADP-specific Phosphofructokinase/Glucokinase conserved region
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLC 1 x GLC
Ligand BGC 1 x BGC
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: I222
Unit cell:
a: 112.677Å b: 88.948Å c: 103.355Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.205
Expression system: Escherichia coli

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Citations

3 review citations

Hypothesis: structures, evolution, and ancestor of glucose kinases in the hexokinase family.
Kawai et al. (2005)
2 more

9 mentions without citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
8 more