› 1u67

X-ray diffraction
3.1Å resolution

Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostagladin H Synthase-1 that Forms Predominantly 11-HPETE.

Released:
DOI: 10.2210/pdb1u67/pdb
PDB entry 1u67 coloured by chain, front view.
PDB entry 1u67 coloured by chain, side view.
PDB entry 1u67 coloured by chain, top view.
Source organism: Ovis aries
Primary publication:
Crystal structure of arachidonic acid bound to a mutant of prostaglandin endoperoxide H synthase-1 that forms predominantly 11-hydroperoxyeicosatetraenoic acid.
Harman CA, Rieke CJ, Garavito RM, Smith WL
J Biol Chem 279 42929-35 (2004)
PMID: 15292194

Function and Biology Details

Reaction catalysed: 
Arachidonate + AH(2) + 2 O(2) = prostaglandin H(2) + A + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Prostaglandin G/H synthase 1 Chain: A
Molecule details ›
Chain: A
Length: 600 amino acids
Theoretical weight: 68.78 KDa
Source organism: Ovis aries
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P05979 (Residues: 1-600; Coverage: 100%)
Gene names: COX1, PTGS1
Sequence domains: Animal haem peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand COH 1 x COH
Carbohydrate polymer : NEW Components: NAG, NDG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand BOG 4 x BOG
Ligand ACD 1 x ACD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 181.879Å b: 181.879Å c: 103.368Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.238 0.311
Expression system: Spodoptera frugiperda

Quick links

Citations

3 review citations

Enzymes of the cyclooxygenase pathways of prostanoid biosynthesis.
Smith et al. (2011)
2 more

4 mentions without citation

Structural and Chemical Biology of the Interaction of Cyclooxygenase with Substrates and Non-Steroidal Anti-Inflammatory Drugs.
Rouzer et al. (2020)
3 more