› 1u5u

X-ray diffraction
2Å resolution

The structure of an Allene Oxide Synthase reveals a novel use for a catalase fold

Released:
DOI: 10.2210/pdb1u5u/pdb
PDB entry 1u5u coloured by chain, front view.
PDB entry 1u5u coloured by chain, side view.
PDB entry 1u5u coloured by chain, top view.
Source organism: Plexaura homomalla
Primary publication:
The structure of coral allene oxide synthase reveals a catalase adapted for metabolism of a fatty acid hydroperoxide.
Oldham ML, Brash AR, Newcomer ME
Proc Natl Acad Sci U S A 102 297-302 (2005)
PMID: 15625113

Function and Biology Details

Reaction catalysed: 
Arachidonate + O(2) = (5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-127557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Allene oxide synthase-lipoxygenase protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 42.92 KDa
Source organism: Plexaura homomalla
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O16025 (Residues: 1-374; Coverage: 35%)
Structure domains: Catalase core domain

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CAMD BEAMLINE GCPCC
Spacegroup: C2
Unit cell:
a: 147.94Å b: 76.679Å c: 77.761Å
α: 90° β: 109.66° γ: 90°
R-values:
R R work R free
0.209 0.196 0.239
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development.
Wasternack C. (2007)
11 more

5 mentions without citation

Oxygen Activation and Radical Transformations in Heme Proteins and Metalloporphyrins.
Huang et al. (2018)
4 more