1u5i Summary


Crystal Structure analysis of rat m-calpain mutant Lys10 Thr

The structure was published by Hosfield, C.M., Elce, J.S., and Jia, Z., in 2004 in a paper entitled "Activation of calpain by Ca2+: roles of the large subunit N-terminal and domain III-IV linker peptides" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.86 Å and deposited in 2004.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Calpain 2, large [catalytic] subunit precursor and Calpain small subunit 1.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calpain 2, large [catalytic] subunit precursor Q07009 (1-700) (CAN2_RAT)search Rattus norvegicussearch 100% 700 89%
B Calpain small subunit 1 Q64537 (88-270) (CPNS1_RAT)search Rattus norvegicussearch < 90% 184 95%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
Q07009 (1 - 700) Calpain 2, large [catalytic] subunit precursor Rattus norvegicus
Q64537 (88 - 270) Calpain small subunit 1 Rattus norvegicus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (Q07009) Penta-EF-hand proteinssearch, Calpain large subunit, middle domain (domain III)search, Calpain large subunit, catalytic domain (domain II)search Jelly Rollssearch, EF-handsearch PF00648: Calpain family cysteine proteasesearch, PF01067: Calpain large subunit, domain IIIsearch, PF13833: EF-hand domain pairsearch
B Penta-EF-hand proteinssearch EF-handsearch EF handsearch, EF-hand domain pairsearch

Chain ID Cellular component (GO) Molecular function (GO) Biological process (GO)
A (Q07009) plasma membranesearch cytoplasmsearch extracellular vesicular exosomesearch cytosolsearch nucleussearch Golgi apparatussearch membrane raftsearch membranesearch intracellularsearch chromatinsearch perinuclear endoplasmic reticulumsearch endoplasmic reticulumsearch dendritesearch pseudopodiumsearch calcium-dependent cysteine-type endopeptidase activitysearch protein bindingsearch calcium ion bindingsearch peptidase activitysearch cytoskeletal protein bindingsearch protein heterodimerization activitysearch hydrolase activitysearch metal ion bindingsearch cysteine-type peptidase activitysearch response to hypoxiasearch proteolysissearch protein autoprocessingsearch myoblast fusionsearch regulation of cytoskeleton organizationsearch cellular response to amino acid stimulussearch blastocyst developmentsearch
B (Q64537) calcium ion bindingsearch proteolysissearch

Chain InterPro annotation
A Peptidase C2, calpain, catalytic domainsearch EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch Peptidase C2, calpain, large subunit, domain IIIsearch Peptidase C2, calpain, domain IIIsearch Peptidase C2, calpain familysearch Calpain-2search
B EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch Calpain small subunit 1search