› 1tzh

X-ray diffraction
2.6Å resolution

Crystal Structure of the Fab YADS1 Complexed with h-VEGF

Released:
DOI: 10.2210/pdb1tzh/pdb
PDB entry 1tzh coloured by chain, front view.
PDB entry 1tzh coloured by chain, side view.
PDB entry 1tzh coloured by chain, top view.
Source organisms:
Primary publication:
Synthetic antibodies from a four-amino-acid code: a dominant role for tyrosine in antigen recognition.
Fellouse FA, Wiesmann C, Sidhu SS
Proc Natl Acad Sci U S A 101 12467-72 (2004)
PMID: 15306681

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210643 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
N-VEGF Chains: V, W
Molecule details ›
Chains: V, W
Length: 102 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15692 (Residues: 214-315; Coverage: 26%)
Gene names: VEGF, VEGFA
Sequence domains: VEGF heparin-binding domain
Structure domains: Cystine-knot cytokines
Fab YADS1 Light Chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 213 amino acids
Theoretical weight: 23.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab YADS1 Heavy Chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 229 amino acids
Theoretical weight: 24.3 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 83.269Å b: 76.269Å c: 112.497Å
α: 90° β: 105.77° γ: 90°
R-values:
R R work R free
0.215 0.212 0.271
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Selecting and screening recombinant antibody libraries.
Hoogenboom HR. (2005)
36 more

14 mentions without citation

A Structural Overview of Vascular Endothelial Growth Factors Pharmacological Ligands: From Macromolecules to Designed Peptidomimetics.
Ye et al. (2021)
13 more